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Survival of Bull Spermatozoa Seeded and Frozen at Different Rates in Egg Yolk-Tris and Whole Milk Extenders

Department of Animal Science, Cornell University, Ithaca, Ny 14853-4801
Department of Animal Science, Cornell University, Ithaca, NY 14853-4801

Six factorially arranged experiments were designed to study effects of seeding, freezing, and thawing rates in whole milk and egg yolk-Tris extenders commonly used for commercial cryopreservation of bull sperm. In these extenders, semen normally is supercooled to –13 or –14°C unless the sperm are seeded. When sperm were supercooled or seeded, either mechanically or with immobilized silver iodide, and frozen to –196°C, the postthaw percentages of motile sperm were 59, 57, and 64%, respectively. Freezing rates of –15, –25, and –35°C/min gave similar sperm survival rates and were superior to –5°C/min. For milk, the critical freezing temperature extended to –75°C before transfer to liquid nitrogen gave good results. For egg yolk-Tris extender, transfer to liquid nitrogen was less critical once –50°C had been attained. Thawing of sperm in water baths at 25 and 45°C gave similar results, and both temperatures were superior to 5°C. The postthaw percentage of motile sperm in egg yolk-Tris was equal or superior to that of sperm frozen in milk. A freezing rate of –15°C/min to –100°C and thawing at 25°C consistently gave good results.

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