JDS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Dairy Science Vol. 76 No. 11 3362-3368
© 1993 by American Dairy Science Association ®
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lu, D. D.
Right arrow Articles by Nielsen, S. S.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Lu, D. D.
Right arrow Articles by Nielsen, S. S.

Assays for Native Plasminogen Activators in Bovine Milk

Dongjin D. Lu 1 and S. Suzanne Nielsen 1

1 Department of Food Science, Purdue University, West Lafayette, IN 47907-1160

Assays for bovine milk plasminogen activators were developed. The colorimetric assay for bovine milk plasminogen activators was based on plasmin cleavage of the synthetic substrate SpectrozymeTM. This substrate cleavage yielded the yellow chromophore p-nitroanilide, which was quantified spectrophotometrically at 405 nm with an ELISA plate reader. Correlation coefficients were >.995 for the linear regions of the standard curves for the assays. Modification of a previously reported casein-plasminogen SDS-PAGE system allowed visualization of plasminogen activators from bovine milk. This system can be used to determine the molecular mass of the milk plasminogen activators and to detect the activity of the plasminogen activators in a sample containing plasminogen activator inhibitors.

Key Words: assays • plaminogen activators • bovine milk

Submitted on November 12, 1992
Accepted on May 19, 1993




This article has been cited by other articles:


Home page
 J DAIRY SCIHome page
L. Wang, K. D. Hayes, and L. J. Mauer
Fluorescent Labeling Study of Plasminogen Concentration and Location in Simulated Bovine Milk Systems
J Dairy Sci, January 1, 2006; 89(1): 58 - 70.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1993 by the American Dairy Science Association ®.