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Journal of Dairy Science Vol. 75 No. 7 1826-1834
© 1992 by American Dairy Science Association ®
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Kinetics and Characteristic of Bovine Neutrophil Alkaline Phosphatase During Acute Escherichia coli Mastitis

R. Heyneman 1 and C. Burvenich 1

1 University of Gent, Casinoplein 24, B-9OOO Gent, Belgium

Alkaline phosphatase activity of isolated bovine blood neutrophils was investigated before and during experimentally induced Escherichia coli mastitis. Activities markedly increased 1 wk after infection in neutrophils of cows suffering from moderate or from severe disease. Elevated neutrophil alkaline phosphatase activity did not correlate with a specific stage of maturating postmitotic neutrophils appearing in circulation during mastitis. Neutrophil alkaline phosphatase from healthy cows before infection and from mastitic cows was characterized by means of thermostability, specific inhibitor patterns, slab gel electrophoresis, and kinetic parameter analysis. The leukocyte enzyme from healthy and mastitic cows displayed very similar characteristics, suggesting that the increase in activity during mastitis is most probably related to the enhanced expression of the normal alkaline phosphatase enzyme.

Key Words: neutrophil leukocytes • alkaline phosphatase • Escherichia coli mastitis

Submitted on September 21, 1991
Accepted on February 14, 1992






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Copyright © 1992 by the American Dairy Science Association ®.