Variations and Distributions of O-Glycosidically Linked Sugar Chains in Bovine -Casein

¹ Laboratory of Animal Products Chemistry, College of Agriculture, Tohoku University, Tsutsumidori-Amamiyamachi, Sendai 981, Miyagi, Japan

The variation and distribution of O-glycosidically linked sugar chains in -casein from bovine mature milk were analyzed by HPLC, fast atom bombardment mass spectrometry, and ¹³C nuclear magnetic resonance. A monosaccharide alditol (N-acetylgalactosaminitol) and four oligosaccharide alditols [a neutral disaccharide (galactosyl-ß1-3N-acetylgalactosaminitol), two acidic trisaccharides (straight chain: N-acetylneuraminyl 2-3galactosyl-ß1-3N-acetylgalactosaminitol and branch chain: galactosyl ß1-3 (N-acetylneuraminyl 2-6)N-acetylgalactosaminitol), and an acidic tetrasaccharide (N-acetylneuraminyl 2-3galactosyl-ß1-3(N-acetylgalactosaminyl 2-6)N-acetylgalactosaminitol] were identified as sugar chains linking on normal bovine -casein. The most dominant sugar chain was an acidic tetrasaccharide. The four oligosaccharide chains were identical to those already found, but N-acetylgalactosamine was a newly identified sugar chain linking on -casein. The distribution of monosaccharide, disaccharide, trisaccharide (straight), trisaccharide (branched), and tetrasaccharide chains were determined by HPLC to be .8, 6.3, 18.4, 18.5, and 56.0%, respectively (means of five -caseins).

Key Words: -casein • sugar chain distribution • fast atom bombardment mass • spectrometry • carbon-13 nuclear magnetic resonance

Submitted on August 26, 1991
Accepted on January 14, 1992

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