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Journal of Dairy Science Vol. 75 No. 5 1192-1196
© 1992 by American Dairy Science Association ®
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Lithium Chloride-Sodium Propionate Agar for the Enumeration of Bifidobacteria in Fermented Dairy Products

Luciane Lapierre 1, P. Undeland 1, and L. J. Cox 1

1 Quality and Safety Assurance Department, Nestec Ltd., Nestlé Research Center, Vers-Chez-les-Blanc, Switzerland

Lithium chloride-sodium propionate agar has been developed for the enumeration of bifidobacteria in fermented dairy products. The medium contains lithium chloride and sodium propionate to inhibit the growth of other lactic acid bacteria Pure cultures of bifidobacteria, lactobacilli, and streptococci were tested for growth in this medium. With one exception, all bifidobacteria were able to grow in this medium and in a nonselective agar with a difference not exceeding .4 log units. However, none of the lactobacilli tested and only one strain each of Streptococcus salivarius ssp. thermophilus and Lactococcus lactis ssp. cremoris grew in lithium chloride-sodium propionate agar. In those cases, the numbers of colonies were lower in lithium chloride-sodium propionate agar by 1.26 and 2.51 log units, respectively, compared with a nonselective agar. Bifidobacteria were also selectively isolated from all fermented milks and cheeses analyzed.

Key Words: enumeration • bifidobacteria • fermented dairy products • lithium chloride-sodium propionate agar

Submitted on June 28, 1991
Accepted on January 14, 1992




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