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Journal of Dairy Science Vol. 75 No. 4 971-975
© 1992 by American Dairy Science Association ®
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The Least Number of Phosphate Groups for Crosslinking of Casein by Colloidal Calcium Phosphate

Takayoshi Aoki 1, Taketoshi Umeda 1, and Yoshitaka Kako 1

1 Department of Biochemical Science and Technology, Faculty of Agriculture Kagoshima University, Kagoshima 890, Japan

Artificial casein micelles were formed with whole human casein at 20 mM calcium, 17 mM phosphate, and 10 mM citrate. The casein micelles disaggregated by 6 M urea were separated by high performance gel chromatography on a TSK-GEL G4000SW column into crosslinked and monomeric fractions. When the crosslinked casein fraction was analyzed by high performance ion-exchange chromatography on a TSK-GEL DEAE-5PW column, a small peak, representing the 3-P component of human ß-casein, and distinct peaks of the 4-P and 5-P components were found. Artificial casein micelles were formed from mixtures of each purified component of human ß-casein and bovine kappa-casein, disaggregated by urea, and separated on a TSK-GEL G4000SW column. The casein aggregates crosslinked by colloidal calcium phosphate were formed in artificial casein micelles of the 3-P and 4-P components. In contrast, no crosslinkage was formed in artificial micelles of the 1-P and 2-P components. The results indicate that at least three phosphate groups are needed for crosslinking of casein by colloidal calcium phosphate.

Key Words: casein micelle • human ßbeta;-casein • calcium phosphate • crosslinking

Submitted on August 12, 1991
Accepted on November 25, 1991




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