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1 Station de Recherches Laitières, Institut National de la Recherche Agronomique, 78352 Jouy-en-Josas, Cedex, France
Ropy strains of Lactococcus lactis ssp. lactis, Lactococcus lactis ssp. cremoris, and Lactobacillus casei ssp. casei produced heteropolysaccharides, when grown in milk and ultrafiltrate, containing primarily galactose and glucose with the former predominating. However, small amounts of mannose, rhamnose, and pentoses were also identified. The amounts of polysaccharide produced ranged from 30 to 600 mg/L of culture medium; they were 50 to 60% higher when strains grew at 25 instead of 30°C. Addition of glucose or sucrose to both milk and ultrafiltrate markedly enhanced exopolysaccharide production and modified the monosaccharide composition with glucose becoming dominant.
Key Words: exopolysaccharides • lactic acid bacteria
Submitted on June 7, 1991
Accepted on October 28, 1991
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