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Journal of Dairy Science Vol. 74 No. 9 2872-2878
© 1991 by American Dairy Science Association ®
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Isolation and Characterization of Monoclonal Antibody Directed Against Bovine alphas2-Casein

Cecilia T. Leung 1, Konrad M. Kuzmanoff 1, and Craig W. Beattie 1

1 Specialized Center for Cancer Research and Education, University of Illinois School of Medicine at Chicago, Chicago 60612

A monoclonal antibody 62-1A of isotype IgM, directed against bovine alphas2-casein, was isolated and characterized. Monoclonal antibody 62-1A recognized bovine alphas2-11P- and alphas2-9P-caseins by indirect solid phase radioimmunoassay and Western blot analysis. Crossreactivity toward native genetic variants (A, B, and C) of alphas1-casein was similar but lower (–60 to 70%) than that for alphas2-11P-casein). Little or no crossreactivity was observed for other bovine milk or serum proteins. Antibody affinity for alphas2-11P-casein was 1.3 x 109/M. As little as 25 ng/ml (.5 ng/well) of alphas2-11P-casein was detected by solid phase radioimmunoassay.

Key Words: monoclonal antibodies • alphas2-casein • milk proteins

Submitted on January 28, 1991
Accepted on April 26, 1991






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Copyright © 1991 by the American Dairy Science Association ®.