JDS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Dairy Science Vol. 74 No. 8 2448-2458
© 1991 by American Dairy Science Association ®
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kehrli, M. E.
Right arrow Articles by Boone, T. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kehrli, M. E., Jr.
Right arrow Articles by Boone, T. C.

Effects of Granulocyte Colony-Stimulating Factor Administration to Periparturient Cows on Neutrophils and Bacterial Shedding

Marcus E. Kehrli Jr. 1, Jesse P. Goff 1, Mark G. Stevens 1, and Thomas C. Boone 1

1 Metabolic Diseases and Immunology Laboratory, National Animal Disease Center, Agricultural Research Service, USDA, Ames, Iowa 50010

Administration of recombinant bovine granulocyte colony stimulatory factor to periparturient dairy cows was evaluated as a method to prevent periparturient immunosuppression. Eleven of 21 cows were experimentally infected with Staphylococcus aureus in one mammary quarter prior to the study. Cows were randomly assigned to four groups in a 2 x 2 factorial design to evaluate the effects of placebo or recombinant bovine granulocyte colony stimulatory factor administration on chronic, subclinically infected and uninfected cows during the periparturient period. Blood neutrophils were isolated and evaluated for phagocytic activities 5 wk before expected parturition through 7 wk postpartum. Administration of recombinant bovine granulocyte colony stimulatory factor (5 µg/kg body weight or placebo subcutaneously beginning 14 d prepartum through 10 d postpartum) resulted in a prepartum and postpartum leukocytosis of 35,600/µl and 53,500/µl, respectively. This was attributed to a mature neutrophilia of 24,010/µl during prepartum and 38,080/µl during postpartum treatment periods (pretreatment baseline = 2330/µl). Mononuclear cell counts averaged 7610/µl during prepartum and 9830/µl during postpartum treatment periods (baseline = 3450/µl). Neutrophil random and directed migration were reduced during recombinant bovine granulocyte colony stimulatory factor treatment compared with placebo or baseline levels. Ingestion of bacteria and cytotoxicity by neutrophils was increased during recombinant bovine granulocyte colony stimulatory factor therapy compared with placebo or baseline levels. Shedding of S. aureus in lacteal secretions was unaffected by recombinant bovine granulocyte colony stimulatory factor treatment. In summary, administration of recombinant bovine granulocyte colony stimulatory factor increased the number and functional activity of neutrophils and prevented some aspects of periparturient immunosuppression in dairy cows.

Key Words: mastitis • hematology • cytokine

Submitted on October 26, 1990
Accepted on April 10, 1991




This article has been cited by other articles:


Home page
 Vet PatholHome page
S. A. Youssef, M. E. Clark, and J. L. Caswell
Effect of Bovine Granulocyte Colony-Stimulating Factor on the Development of Pneumonia Caused by Mannheimia haemolytica
Vet. Pathol., November 1, 2004; 41(6): 649 - 657.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
G. B. Mitchell, B. N. Albright, and J. L. Caswell
Effect of Interleukin-8 and Granulocyte Colony-Stimulating Factor on Priming and Activation of Bovine Neutrophils
Infect. Immun., April 1, 2003; 71(4): 1643 - 1649.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1991 by the American Dairy Science Association ®.