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Journal of Dairy Science Vol. 74 No. 6 1773-1778
© 1991 by American Dairy Science Association ®
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Specificity, Inhibitory Studies, and Oligosaccharide Formation by ß-Galactosidase from Psychrotrophic Bacillus subtilis KL88

Khalid A. A. Rahim 1 and Byong H. Lee 1

1 Department of Food Science and Agricultural Chemistry, Macdonald Campus of McGill University, Ste. Anne de Bellevue, PQ, Canada H9X 1C0

ß-Galactosidase from psychrotrophic Bacillus subtilis KL88 was specific to the ß-D-glycosidic linkage normally present in lactose. The enzyme was completely inhibited by transition metal ions (Cu2+, Fe3+, Fe2+, Zn2+) and partially inhibited by high concentrations of glucose and galactose as well as Ca2+. It was activated by most of the alkaline earth metal ions (NA+, K+, Li+). Oligosaccharides were formed at the different levels of lactose concentrations reaching more than 20% for high lactose concentration (20%). Three types of oligosaccharides were formed in significant concentrations detected by HPLC analysis.

Key Words: oligosaccharide formation • ßbeta;-galactosidase • psychrotrophic • Bacillus subtilis KL88

Submitted on May 21, 1990
Accepted on September 21, 1990




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[Abstract] [Full Text]


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