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Journal of Dairy Science Vol. 74 No. 5 1544-1549
© 1991 by American Dairy Science Association ®
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Isolation of Staphylococcus aureus from Sites Other than the Lactating Mammary Gland

J. S. Matos 1, D. G. White 1, R. J. Harmon 1, and B. E. Langlois 1

1 University of Kentucky, Lexington 40546-0215

The body sites of 163 heifers, bedding and feedstuff samples, flies, and hands and nares of the research personnel were sampled in order to determine the sources of Staphylococcus aureus in a dairy herd other than the lactating mammary gland. Lesions on the udder of lactating animals and the air in the milking parlor were also sampled. Staphylococci isolated from bedding samples were identified as to species. Staphylococcus aureus was isolated from all sources examined except flies. An enrichment procedure was necessary for isolating S. aureus from two bedding samples although other Staphylococcus species were present in high numbers. The designation "environmental staphylococci" is proposed for Staphylococcus species that were apparently free-living in the environment.

Key Words: Staphylococcus aureus • mammary gland • body sites

Submitted on July 30, 1990
Accepted on December 10, 1990




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