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Journal of Dairy Science Vol. 74 No. 5 1454-1460
© 1991 by American Dairy Science Association ®
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Transformation of Dairy Leuconostoc Using Plasmid Vectors from Bacillis, Escherichia, and Lactococcus Hosts

Herb A. Wyckoff 1, W. E. Sandine 1, and Jeffery K. Kondo 2

1 Department of Microbiology, Oregon State University, Corvallis 97331-3804
2 Marshall Products, Madison, WI 53713

Conditions that allow efficient genetic transformation of dairy Leuconostoc by electroporation were determined. The technique allowed transformation of Leuconostoc mesenteroides ssp. cremoris, Leuconostoc mesenteroides ssp. dextranicum, and Leuconostoc lactis with plasmid pNZ12. Optimized conditions resulted in transformation efficiencies of up to 2 x 106/µg of pNZ12 DNA for L. cremoris 44-4. Further, cloning vectors pNZ18, pGK12, pGK13, pGL3, pBD64, pGB301, pDB101, and pAMß1 from lactococcal, Bacillus, and Escherichia hosts were introduced and maintained by L. cremoris 44-4, extending the range of vectors functional in Leuconostoc.

Key Words: Leuconostoc • transformation • plasmids

Submitted on August 9, 1990
Accepted on December 28, 1990




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Copyright © 1991 by the American Dairy Science Association ®.