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Journal of Dairy Science Vol. 74 No. 4 1235-1242
© 1991 by American Dairy Science Association ®
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Antigenic Homology Among Gram-Negative Organisms Isolated from Cattle with Clinical Mastitis

Jeff Tyler 1, Harold Spears 1, James Cullor 2, Wayne Smith 2, Renee Nelson 1, and James Martin 3

1 Department of Large Animal Surgery and Medicine, Auburn University, AL 36849-5522
2 Department of Clinical Pathology, School of Veterinary Medicine, University of California, Davis 95616
3 C. S. Roberts Veterinary Diagnostic Laboratory, Auburn, AL 38631

This study examined the degree of serologic homology among mastitis pathogens. Antibodies were raised against the Rc mutant, Escherichia coli O111:B4 (strain J5) and affinity purified against lipopolysaccharide derived from the Ra mutant, Salmonella typhimurium TV119. These antibodies reacted with a battery of unrelated Gram-negative bacteria in whole cell ELISA. Bacteria with strong cross-reactions included a heterologous, smooth E. coli, Salmonella dublin, S. typhimurium, Salmonella newport, and Pseudomonas aeruginosa. Recognition of Klebsiella pneumoniae and Bordetella bronchisepticum was observed, but reactions were weaker than with the other isolates. The reduced recognition of these isolates probably reflects a masking effect of the bacterial capsule and variations in lipopolysaccharide structure. The polyclonal antibody did not recognize a Gram-positive isolate, Staphylococcus aureus.

These immunoglobulins were then tested using whole cell ELISA against a panel of bacteria recovered from the mammary glands of cattle with clinical mastitis, Marked reactivity was noted against a variety of Gram-negative pathogens. Gram-positive isolates had lower recognition by Gram-negative core antigen specific immunoglobulin. The results suggest immunization with rough mutant bacteria may have broad application in the prevention of coliform mastitis.

Key Words: mastitis • Gram-negative homology

Submitted on August 20, 1990
Accepted on November 19, 1990




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A. Chaiyotwittayakun, J. L. Burton, P. S. D. Weber, K. Kizilkaya, F. F. Cardoso, and R. J. Erskine
Hyperimmunization of Steers with J5 Escherichia coli Bacterin: Effects on Isotype-Specific Serum Antibody Responses and Cross Reactivity with Heterogeneous Gram-Negative Bacteria
J Dairy Sci, October 1, 2004; 87(10): 3375 - 3385.
[Abstract] [Full Text] [PDF]


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Copyright © 1991 by the American Dairy Science Association ®.