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Journal of Dairy Science Vol. 74 No. 2 426-428
© 1991 by American Dairy Science Association ®
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A Convenient Method for Differentiation of Coagulase-Negative Staphylococci Isolated from Bovine Mammary Glands

J. L. Watts 1, C. H. Ray 1, and P. J. Washburn 1

1 Mastitis Research Laboratory, Hill Farm Research Station, Louisiana Agricultural Experìment Station, Louisiana State University Agricultural Center, Route 1, Box 10, Homer 71040

The utility of trehalose-mannitol broth and arabinose-cellobiose broth for identification of Staphylococcus epidermidis and novobiocin-resistant staphylococci was determined using 236 coagulase-negative staphylococci isolated from bovine mammary glands. None of the 49 S. epidermidis strains was positive in trehalose-mannitol broth; whereas, all strains of Staphylococcus hyicus, Staphylococcus chromogenes, Staphylococcus haemolyticus, Staphylococcus hominis, Staphylococcus warneri, and Staphylococcus simulans were positive. Of the novobiocin-resistant staphylococcal species, only Staphylococcus saprophyticus was negative in arabinose-cellobiose broth. Except for one strain of Staphylococcus sciuri and one strain of Staphylococcus kloosii, all remaining strains of novobiocin-resistant staphylococcal species were positive in arabinose-cellobiose broth. Results indicate that trehalose-mannitol broth is an acceptable method for identification of S. epidermidis isolated from bovine mammary glands. Furthermore, arabinose-cellobiose broth is a useful method of screening for novobiocin-resistant staphylococci.

Key Words: coagulase-negative staphylococci • identification • bovine mastitis

Submitted on July 2, 1990
Accepted on September 17, 1990






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Copyright © 1991 by the American Dairy Science Association ®.