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Journal of Dairy Science Vol. 74 No. 12 4183-4188
© 1991 by American Dairy Science Association ®
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Microbiological Results from Milk Samples Obtained Premilking and Postmilking for the Diagnosis of Bovine lntramammary Infections

Philip W. Sears 1, David J. Wilson 1, Ruben N. Gonzalez 1, and DALE D. HANCOCK 2

1 Quality Milk Promotion Services, Cornell University, Ithaca, NY 14850
2 College of Veterinary Medicine, Washington State University, Pullman 99164

Bacteriological culture results were compared between 336 pairs of quarter milk samples collected premilking and postmiking. Using a positive result on either premilking or postmilking samples as the definitive diagnosis, premilking sampling sensitivity was 91% for Staphylococcus aureus, 91% for coagulase-negative staphylococci, and 97% for Streptococcus other than agalactiae. Postmilking sampling sensitivities were 81, 45, and 58%, respectively, for the same pathogens. Requiring both premilking and postmilking samples for the definitive diagnosis, specificities were 92, 86, and 95% for premilking sampling alone and 96, 98, and 99% for postmilking sampling alone. Such differences in specificity would result in major differences in predictive value of a positive culture for herds with a low prevalence. Multiple isolates were significantly more common from premilking samples.

Key Words: microbiology • mastitis • culture samples • intramammary infections

Submitted on May 2, 1991
Accepted on July 12, 1991




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