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Journal of Dairy Science Vol. 74 No. 11 3731-3740
© 1991 by American Dairy Science Association ®
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Isolation of Monoclonal Antibodies Monospecific for Bovine ß-Lactoglobulin

Konrad M. Kuzmanoff 1 and Craig W. Beattie 1

1 Specialized Center for Cancer Research and Education, University of Illinois School of Medicine at Chicago, Chicago 60612

Monoclonal antibodies were generated against purified bovine ß-lactoglobulin A. Six antibodies reactive only with ß-lactoglobulin were selected. At least one of the antibodies appeared to be species monospecific for bovine ß-lactoglobulin. The remaining five antibodies recognized proteins in caprine and porcine whey fractions. One monoclonal antibody (59-1) exhibited a distinct 2:l preference for ß-lactoglobulin B over A at near neutral pH (7.5). These antibodies should prove extremely useful adjuncts in structural studies of bovine ß-lactoglobulin.

Key Words: bovine • ßbeta;-lactoglobulin • monoclonal antibodies

Submitted on February 14, 1991
Accepted on June 21, 1991






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