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Journal of Dairy Science Vol. 74 No. 1 46-49
© 1991 by American Dairy Science Association ®
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Separation of Proteinase from ß-Galactosidase of Psychrotrophic Bacillus subtilis KL88

Khalid A. A. Rahim 1 and Byong H. Lee 1

1 Department of Food Science and Agricultural Chemistry, Macdonald College of McGill University, Ste. Anne de Bellevue, PQ, H9X 1C0, Canada

To investigate the possibility of producing proteinase-free ß-galactosidase from Bacillus subtilis, the fast protein liquid chromatography system was used as a means for the rapid separation of ß-galactosidase. One step ion-exchange chromatography with the fast protein liquid chromatography system resulted in rapid separation of proteinase from ß-galactosidase with a yield of 75% in 30 min.

Key Words: ßbeta;-galactosidase • proteinase • Bacillus subtilis

Submitted on March 5, 1990
Accepted on August 13, 1990






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Copyright © 1991 by the American Dairy Science Association ®.