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Journal of Dairy Science Vol. 73 No. 8 2044-2053
© 1990 by American Dairy Science Association ®
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Cloning of Phage Resistance Genes from Lactococcus Lactis ssp. cremoris KH

M. E. Sanders 1 and J. W. Shultz 2

1 Marschall Dairy Group, Miles Inc., PO Box 932, Elkhart, IN 46515
2 Promega, 2800 South Fish Hatchery Road, Madison. WI 53711-5305

A 17.5-kb plasmid conferring restriction and modification-type phage resistance in Lactococcus lactis ssp. cremoris KH was transformed into Lactococcus Lactis LM0230 and cloned onto an origin of replication-deficient vector directly into L. lactis. Expression of phage resistance was seen in L. lactis but not in E. coli. A restriction map was generated of the cloned plasmid, and the region encoding phage resistance was localized by insertional inactivation and deletion analysis.

Key Words: phage resistance • Lactococcus • cloning

Submitted on November 20, 1989
Accepted on February 26, 1990




This article has been cited by other articles:


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 Appl. Environ. Microbiol.Home page
A. Forde, C. Daly, and G. F. Fitzgerald
Identification of Four Phage Resistance Plasmids from Lactococcus lactis subsp. cremoris HO2
Appl. Envir. Microbiol., April 1, 1999; 65(4): 1540 - 1547.
[Abstract] [Full Text]


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