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Journal of Dairy Science Vol. 73 No. 7 1690-1693
© 1990 by American Dairy Science Association ®
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Effect of Sample Size and Concentration on High Performance Liquid Chromatographic Determination of Vitamin A in Skim Milk Powder

John McNeill 1, Michael Hincks 1, and Yukio Kakuda 2

1 ORTECH International, Sheridan Park Research Community, Mississauga, Ontario, L5K 1B3
2 Food Science Department, University of Guelph, Guelph, Ontario, N1G 2W1

A previously established HPLC method for the determination of vitamin A in fluid milk was applied to fortified skim milk powder. Five concentrations of powder (100, 200, 300, 400, and 500 g powder/L distilled water) were extracted to determine which would produce the highest recovery. The results indicated that a 10% concentration produced the highest level of vitamm A. Exhaustive extraction using a 10% concentration of powder did not increase the recovery over that of a single extraction. The effect of total sample weight on method recovery and representative sampling was determined by extracting 10% concentrations containing 5, 10, 20, 50, and 100 g of powder. A minimum of 50 g of powder was required to ensure the highest recovery and precision.

Key Words: skim milk powder • vitamin A • high performance liquid chromatography

Submitted on November 20, 1989
Accepted on February 1, 1990






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Copyright © 1990 by the American Dairy Science Association ®.