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Journal of Dairy Science Vol. 73 No. 7 1684-1689
© 1990 by American Dairy Science Association ®
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Isolation and Characterization of Polysaccharide-Producing Bacteria from Kefir Grains

Haruhiko Yokoi 1, Takashi Watanabe 1, Yoshitsugu Fuji 1, Takahiro Toba 2, and Susumu Adachi 2

1 Sumitomo Heavy Industries, Ltd., Hiratsuka Research Laboratory, Yuhigaoka 63-30 Hiratsuka, Kanagawa 254, Japan
2 Laboratory of Animal Products Technology, College of Agriculture, Tohoku University, Tsutsumidori-Amamiyacho 1-1, Sendal 980, Japan

Five polysaccharide-producing bacteria, which grew actively in a newly developed whey media, were isolated from kefir grains. About 300 to 400 mg of polysaccharide/L of broth were produced with 65 to 85% excreted into the medium. The polysaccharide had equal amounts of glucose and galactose and two main activity peaks with molecular weights of 1.0 x 106 to 4.0 x 106.

Key Words: polysaccharide-producing bacteria • isolation • kefir grains

Submitted on July 7, 1989
Accepted on January 24, 1990






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Copyright © 1990 by the American Dairy Science Association ®.