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Journal of Dairy Science Vol. 73 No. 4 881-886
© 1990 by American Dairy Science Association ®
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Whey-Based Bacteriophage Inhibitory Italian Bulk Starter Medium

S. N. Rajagopal 1, W. E. Sandine 1, and J. W. Ayres 2

1 Department of Microbiology
2 College of Pharmacy, Oregon State University, Corvallis 97331-3804

Six commercially available bulk starter media used for growing rod-coccus thermophilic lactic acid bacteria (e.g., Lactobacillus bulgaricus and Streptococcus thermophilus) were compared. The media were inoculated at the manufacturer's recommended rate with freshly thawed frozen concentrated thermophilic bulk set cultures obtained from two culture supply houses. Media were tested for bacteriophage inhibition and acid-producing activity of the cells grown in each medium. Cells from the media were rated for thermophilic acid-producing activity by summing the differences between the pH of an uninoculated and 1 and 2% inoculated NDM over a temperature profile simulating Mozzarella cheese manufacture. An experimental whey-based thermophilic bulk starter medium also was evaluated in comparison with those commercially available.

Key Words: bulk starter medium • bacteriophage • Italian cheese

Submitted on November 18, 1988
Accepted on October 13, 1989






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