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Journal of Dairy Science Vol. 73 No. 2 308-313
© 1990 by American Dairy Science Association ®
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Homogeneity of Sulfhydryl Oxidase Preparations Obtained by Transient Covalent Affinity Chromatography

Violeta G. Janolino 1 and Harold E. Swaisgood 1

1 Department of Food Science, Southeast Dairy Foods Research Center, North Carolina State University, Raleigh 27695-7624

Bovine milk sulfhydryl oxidase was prepared by transient covalent affinity chromatography on a cysteinylsuccinamidopropyl glass matrix. The homogeneity of this preparation was examined by immunofixation electrofocusing, SDS-PAGE, and size exclusion chromatography in 6 M guanidinium chloride. The major contaminant of this preparation, prepared from whey diafiltered with a 1000 kdal exclusion limit membrane, was IgG. ImmunogJobulin M could not be detected. Presence of IgG in these sulfhydryl oxidase preparations suggests an interaction between IgG and either skim milk membrane vesicles or sulfhydryl oxidase.

Key Words: sulfhydryl oxidase • covalent affinity chromatography • immunoglobulin

Submitted on May 8, 1989
Accepted on August 15, 1989






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Copyright © 1990 by the American Dairy Science Association ®.