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Journal of Dairy Science Vol. 73 No. 2 299-307
© 1990 by American Dairy Science Association ®
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Growth of Bifidobacteria and Their Enzyme Profiles

Marie-Line Desjardins 1, Denis Roy 1, and Jacques Goulet 2

1 Food Research and Development Center, Saint-Hyacinthe, Québec, Canada J2S 8E3
2 Laval University, Québec, PQ Canada G1K 7P4

Bifidobacteria of human origin (22 strains) were evaluated for their potential use in yogurt-type products. Strains were grouped according to their growth rate in 12% reconstituted skim milk. Among the strains that grew quickly and produced acid in milk rapidly, the ratio of acetic: lactic acid varied from .4:1.0 to 3.0:1.0 Enzyme profiles of all strains were determined with the API ZYM system. All bifidobacteria possessed alpha- and ß-galactosidases and alpha-glucosidase activities. A harmful enzyme, ß-glucuronidase, was detected in only one strain of bifidobacteria tested. The faster growing strains also possessed high leucine aminopeptidase activity.

Key Words: Bifidobacteria • growth • enzyme profiles

Submitted on May 19, 1989
Accepted on August 7, 1989




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