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Journal of Dairy Science Vol. 73 No. 10 2929-2933
© 1990 by American Dairy Science Association ®
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Interlaboratory Variation in a Diaminopimelic Acid Assay: Influence on Estimated Duodenal Bacterial Nitrogen Flow

P. H. Robinson 1, G. Coto 2, M. D. Stern 3, and D. M. Veira 4

1 Department of Animal Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5
2 Institute of Animal Science, Calle 30, 768-1, Nuevo Vedado, La Habana Cuba
3 Department of Animal Science, University of Minnesota, St. Paul 55108
4 Animal Research Centre, Agriculture Canada Research Branch, Neatby Building, Ottawa, Ontario, Canada K1A 0C6

Samples of ruminal bacteria and duodenal digesta were collected from two dairy cows fed a 65% forage diet. Samples were sent blind to four laboratories for diaminopimelic acid analysis. Analyzed values differed among laboratories within sample type, and concentrations ranked as follows: laboratory D greater than laboratory A greater than laboratory B greater than laboratory C. Consideration of differences in actual procedures used among laboratories resulted in several hypotheses to explain some of the interlaboratory variation. Using diaminopimelic acid values from each laboratory to estimate duodenal bacterial nitrogen flow showed that laboratory D estimated a 17% higher flow than the average for laboratories A, B, and C, which were similar.

Key Words: bacterial nitrogen flow • diaminopimelic acid • rumen bacteria

Submitted on February 2, 1990
Accepted on May 14, 1990




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J. C. Marini, D. G. Fox, and M. R. Murphy
Nitrogen transactions along the gastrointestinal tract of cattle: A meta-analytical approach
J Anim Sci, March 1, 2008; 86(3): 660 - 679.
[Abstract] [Full Text] [PDF]


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