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Journal of Dairy Science Vol. 73 No. 10 2697-2701
© 1990 by American Dairy Science Association ®
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An Enzymatic Method for Determination of Formate Production by Streptococcus thermophilus

Pablo F. Perez 1, Graciela L. De Antoni 1, and M. Cristina Añon 1

1 Centro de Investigación y Desarrollo en Criotecnología de Alimentos, Universidad Nacional de La Plata, Calle 47 y 116 (1900) La Plata, Argentina

An enzymatic determination of formate in milk samples using formate dehydrogenase (EC 1.2.1.2) was tested. The replacement of HClO4 by TCA in the deproteinization step lead to faster determinations, and no deproteinization was required for samples in culture medium. The method was applied to study formate production by Streptococcus thermophilus ATCC 19258 grown in milk and culture media. Results obtained with the method show that formate levels are higher in milk than in the culture medium.

Key Words: enzymatic assay • yogurt • Streptococcus thermophilus

Submitted on October 4, 1989
Accepted on March 29, 1990






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Copyright © 1990 by the American Dairy Science Association ®.