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Journal of Dairy Science Vol. 73 No. 1 41-44
© 1990 by American Dairy Science Association ®
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An Analytical Approach to the Evaluation of Heat Damage In Commercial Milks

Ella Pagliarini, Stefania Iametti, Claudio Peri and Franco Bonomi

Universita' degli Studi di Milano, Italia

Various samples of pasteurized, UHT, and in-bottle sterilized milk have been analyzed, and two indices of heat damage determined: 1) soluble serum protein as percentage of total proteins and 2) protein surface hydrophobicity determined by a fluorescence test, which enables the number and affinity of bonds between protein hydrophobic sites and fluorophor 8-aniline naphthalene 1-sulfonate to be evaluated.

Results show that the soluble serum protein index permits a definite distinction to be made between pasteurized and sterilized milk but not between UHT and in-bottle sterilized milk. However, the protein surface hydrophobicity index enables a distinction to be made between UHT-treated and in-bottle sterilized milk. In a plot of PSH versus SSP indices, separate areas are identified for raw, pasteurized, UHT, and in-bottle sterilized milk. The combination of the two indices makes it possible to obtain a reliable a posteriori indication of the type and severity of heat treatment the milk has undergone. The possibility of identifying improperly treated milks is also discussed.




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T. L. Mikkelsen, H. Frokiaer, C. Topp, F. Bonomi, S. Iametti, G. Picariello, P. Ferranti, and V. Barkholt
Caseinomacropeptide Self-Association is Dependent on Whether the Peptide is Free or Restricted in {kappa}-Casein
J Dairy Sci, December 1, 2005; 88(12): 4228 - 4238.
[Abstract] [Full Text] [PDF]




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Copyright © 1990 by the American Dairy Science Association ®.