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Journal of Dairy Science Vol. 72 No. 9 2421-2426
© 1989 by American Dairy Science Association ®
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Effect of Inoculation Rate of Selected Strains of Lactic Acid Bacteria on Fermentation and In Vitro Digestibility of Grass-Legume Forage1

J. H. Harrison2, S. O. Soderlund3 and K. A. Loney

Department of Animal Sciences, Washington State University, Dairy Forage Facility, Puyallup 98371

2 Corresponding Author: 7612 Pioneer Way, WSU Puyallup Research and Extension Center, Puyallup 98371.

ABSTRACT

Grass-legume forage was used to evaluate the effect of inoculation rate of selected strains of lactic acid bacteria on fermentation and in vitro digestibility during 57 d of ensiling. Chopped forage (DM = 28%) was ensiled in 4 to 6-kg quantities and treated as: 1) control, 103 epiphytic lactic acid bacteria; 2) 105 added lactic acid bacteria; and 3) 106 added lactic bacteria/g of wet forage. Samples were obtained for analyses on d 0, 1, 2, 3, 6, 10, 14, 29, and 57 of fermentation. Treated silages were observed to have: 1) greater quantities of lactic acid bacteria, 2) a greater proportion of homofermentative lactic acid bacteria, and 3) lactic acid bacteria with greater biological activity. Addition of each amount of lactic acid bacteria: 1) increased the rate of utilization of water-soluble carbohydrate and decline in pH, 2) limited the formation of NH3 N, and 3) increased the in vitro digestibility of DM and ADF. No differences were observed in the lactic acid content of the silages after 57 d of fermentation.

FOOTNOTES

1 College of Agriculture and Home Economics Research Center, Washington State University, Pullman. Project 0724.

3 Pioneer Hi-Bred International, Inc., Microbial Genetics Division, Johnston, IA 50131-0038.

4 Pioneer® brand 1174 water-soluble silage inoculant: dried Lactobacillus plantarum fermentation product, dried Streptococcus faecium product and dried whey. Pioneer Hi-Bred International, Inc., Johnston, IA 50131.






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Copyright © 1989 by the American Dairy Science Association ®.