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Effect of Chemical Treatment on In Vitro and In Situ Degradation of Canola Meal Crude Protein

Department of Animal Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5

ABSTRACT

Effect of chemical treatment on in vitro and in situ digestion of canola meal was investigated. Canola meal was treated by spraying with HCl, acetic acid, formic acid, or propionic acid at concentrations of 2.5 and 5% (vol/wt) followed by drying at 105°C. All acid treatments decreased CP solubility of canola meal in a borate-phosphate buffer solution. In vitro NH₃ production was also decreased by acid treatment of canola meal. Chemical treatment reduced CP digestion by protease enzyme, as reflected by higher residual CP in incubation media for all incubation times. Chemical treatment increased residual CP twofold at 8 h and threefold at 16 and 24 h of in situ incubation. Estimated true digestibility of canola meal CP was not influenced by acetic acid, formic acid, or propionic acid treatment but was reduced by HCl treatment. Acid treatment reduced ruminal degradability of canola meal CP and, with the exception of HCl, did not depress estimated intestinal CP digestibility.

¹ Current address: Agriculture Canada Research Station, PO Box 20280, Fredericton, NB, E3B 4Z7 Canada.