HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Killian, G. Articles by Dunlap, D. Search for Related Content PubMed PubMed Citation Articles by Killian, G. Articles by Dunlap, D.

Evaluation of Butylated Hydroxytoluene as a Cryopreservative Added to Whole or Skim Milk Diluent for Bull Semen¹

Dairy Breeding Research Center, The Pennsylvania State University, University Park 16802

ABSTRACT

Effect of five concentrations of butylated hydroxytoluene on the percent motility and percent intact acrosomes was evaluated for bull sperm frozen and thawed in whole and skim milk diluents. Twelve ejaculates were frozen in .5-ml straws in moving nitrogen vapor after each ejaculate was split among the five butylated hydroxytoluene treatments within the two diluents. Prefreeze and postthaw percent motility and postthaw percent intact acrosomes were evaluated at 0 and 4 h. Before freezing, sperm motilities were similar in whole or skim milk diluents with and without butylated hydroxytoluene. Upon thawing, sperm motility was 10 percentage points higher in whole milk diluent containing .5 and .75 mM butylated hydroxytoluene than in samples without it. After 4 h, percentage motile sperm was similar among all whole milk treatments. Sperm motility was similar among all skim milk treatments at both 0 and 4 h. Percent intact acrosomes were similar among all treatments for each diluent. Oxidation in whole milk diluent stored at 4°C for 1 wk was reduced in the presence of 1.0, 2.0, and 4.0 mM butylated hydroxytoluene.

¹ Journal Series Number 7952, Pennsylvania Agricultural Experiment Station.

This article has been cited by other articles:

				M. R. Fernandez-Santos, F. Martinez-Pastor, V. Garcia-Macias, M. C. Esteso, A. J. Soler, P. Paz, L. Anel, and J. J. Garde Sperm Characteristics and DNA Integrity of Iberian Red Deer (Cervus elaphus hispanicus) Epididymal Spermatozoa Frozen in the Presence of Enzymatic and Nonenzymatic Antioxidants J Androl, March 1, 2007; 28(2): 294 - 305. [Abstract] [Full Text] [PDF]