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Quantification of Apolipoprotein A-1 in Cow Serum by Single Radial Immunodiffusion

Laboratoire des Maladies Métaboliques, INRA, Theix, 63 122 CEYRAT and UA CNRS 524, Laboratoire de Biochimie, Faculté de Médecine, Saint-Antoine 75 571 PARIS, Cedex 12, France

ABSTRACT

A single radial immunodiffusion assay was developed to determine the concentration of the major apolipoprotein A-I in bovine serum. Assay was performed using agarose gel plates with monospecific rabbit antiserum. Standards made with bovine high density lipoproteins or serum 10 times diluted were applied in 3-µl amounts to each well in the gel. The assay working range was 2 to 22 mg of apolipoprotein A-I. The intraassay and interassay CV were 3.7 and 4.9%, respectively. Using protein A-I. The intraassay and interassay CY were 3.7 and 4.9%, respectively. Using radial immunodiffusion assay, 23 serum samples from cows at various stages of lactation were analyzed. Apolipoprotein A-I was significantly increased in mid-lactation compared with concentrations in early lactation or dry period.

¹ INRA, Theix.

² Current address: INTA, EEA Rafaela, 2300 Rafaela, Argentina.

³ Faculté de Médecine Saint-Antoine, Paris.