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Nisin Inhibits Several Gram-Positive, Mastitis-Causing Pathogens¹

Department of Nutrition and Foods Sciences, Utah State University, Logan 84322-8700

² Corresponding author.

ABSTRACT

Organisms known to cause bovine mastitis, Enterococcus faecalis ssp. liquefaciens ATCC 27959, Staphylococcus aureus ATCC 29740, Streptococcus agalactiae ATCC 27956, Streptococcus equinus ATCC 27960, Streptococcus dysgalactiae ATCC 27957, Streptococcus uberis ATCC 27958, and the neotype Staphylococcus epidermidis ATCC 14990 were examined for their susceptibility to die small peptide antibiotic, nisin. Using a disc assay, minimum inhibitory concentrations of nisin ranged from 10 to 250 µg/ml among the strains. Examination of die antimicrobial effect of 50 µg/ml nisin in milk showed nisin inhibited all gram-positive pathogens tested.

¹ This paper is published with the approval of the Director of the Utah Agricultural Experiment Station, Utah State University, Logan as Journal Paper Number 3752.

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