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Journal of Dairy Science Vol. 72 No. 12 3273-3279
© 1989 by American Dairy Science Association ®
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Lysis of Oocytes by Bovine Sperm and Seminal Plasma1

A. Ijaz, A. G. Hunter and M. Ayoub

Department of Animal Science and Large Animal Clinical Sciences, University of Minnesota, St. Paul 55108

ABSTRACT

This study examined the lytic activity of bovine seminal plasma on zona-free oocytes commonly used to assess bovine capacitanon. Exposure of hamster oocytes (intact or zona-free) to undiluted seminal plasma resulted in lysis within 5 min. With intact oocytes, 1:5 to 1:20 seminal plasma resulted in swelling of the oocytes. With zona-free oocytes, seminal plasma (1:0 to 1:1000) resulted in lysis within 1 min to 3 h depending on dilution. Heating seminal plasma to inactivate complement did not reduce lytic activity, but boiling destroyed it. Lytic activity was present in seminal plasma from vasectomized bulls and in seminal vesicle fluid. After elution of seminal plasma through Sephadex G-200, lytic activity was only associated with the fraction containing proteins of 200,000 to 45,000 dal. Lytic activity remained with washed capacitated bull sperm only when 107 sperm/ml or more were coincubated with zona-free oocytes at 37°C for 3 h. In conclusion, both bovine seminal plasma and capacitated sperm were lytic in the zona-free hamster oocyte assay. The results may explain why so few sperm are normally found at the site of fertilization.

FOOTNOTES

1 Published as Paper Number 16,918 of the Scientific Journal Series of the Minnesota Agricultural Experiment Station on research conducted under Minnesota Agricultural Experiment Station Project Number 72 supported by Hatch funds.






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Copyright © 1989 by the American Dairy Science Association ®.