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Milk Protein Typing of Bovine Mammary Gland Tissue Used to Generate a Complementary Deoxyribonucleic Acid Library

Department of Animal Science, University of California, Davis, Davis 95616

ABSTRACT

The milk protein genotype of a mammary gland tissue that was used to generate a cDNA library at the University of California, Davis was typed by PAGE. Casein and whey proteins were extracted from the mammary tissue and typed utilizing fast mini-gel procedures that provide excellent resolution of the milk proteins. The genotype of the mammary tissue was classified as -casein AB, ß-casein A2A1, _s1-casein BB, ß-lactoglobulin AA, and -lactalbumin BB. The genes -casein A, ß-casein A2, ß-lactoglobulin A, and -lactalbumin B that have been cloned from this cDNA library coincide with those in the above tissue genotype with the exception of the recently reported cloning of the _s1-casein A gene. The gene frequencies for die casein genetic variants for three breeds of dairy cattle is presented and discussed in relation to the low frequency (.3%) of the _s1-casein A in the population.