HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Schoonderwoerd, M. Articles by Misra, V. Search for Related Content PubMed PubMed Citation Articles by Schoonderwoerd, M. Articles by Misra, V.

Detection and Quantitation of Pea and Soy-Derived Proteins in Calf Milk Replacers

Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 0W0, Canada

ABSTRACT

Preruminant calves on several farms had diarrhea nonresponsive to treatment and were doing poorly, despite being fed a high quality calf milk replacer. Because these reconstituted milk replacers always had a sediment, they were suspected of containing insoluble nonmilk-derived proteins. Microscopic examination of the milk replacer, however, did not show any evidence of starch granules. We therefore analyzed the samples by SDS PAGE. We were able to identify and quantitate pea protein in calf milk replacers in which all the protein was supposedly milk-derived. We were also able to differentiate polypeptides derived from pea and soy. We concluded that PAGE is a sensitive technique for detecting nonmilk-derived proteins in calf milk replacers.

¹ Department of Veterinary Pathology (present address: Alberta Agriculture, Animal Health Division, PO BOX 8070, Edmonton, Alberta, T6H 4P2, Canada).

² Department of Veterinary Microbiology.