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Cellulose Acetate and Polyacrylamide Gel Electrophoresis for Quantification of Milk Protein Fractions¹

Department of Animal Science

P. B. Addis

Department of Food Science and Nutrition

R. Jenness²

Department of Biochemistry, University of Minnesota, St. Paul 55108

ABSTRACT

Cellulose acetate electrophoresis and PAGE techniques were compared for fractionation and quantification of milk proteins. Protein bands were stained with Ponceau-S and aniline blue black in the cellulose acetate electrophoresis and PAGE, respectively. _s1-Casein and ß-casein absorbed almost equal quantities of Ponceau-S per unit weight, whereas ß-casein absorbed more aniline blue black per unit than did _s1-casein. ß-Lactoglobulin, -lactalbumin, and bovine serum albumin absorbed equal amounts of Ponceau-S per unit but differed in absorption of aniline blue black. It was concluded that cellulose acetate electrophoresis was the method of choice for rapid fractionation and quantification of milk proteins.

¹ Scientific Journal Series Paper Number 15,854, Minnesota Agricultural Experiment Station.

² Present address: Primate Research Institute, New Mexico State University, Holloman Air Force Base 88330.