HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Silva, I. D. Articles by Jain, N. C. Search for Related Content PubMed PubMed Citation Articles by Silva, I. D. Articles by Jain, N. C.

Phagocytic and Nitroblue Tetrazolium Reductive Properties of Bovine Neutrophils for Mammary Pathogens

Department of Clinical Pathology, School of Veterinary Medicine, University of California, Davis 95616

ABSTRACT

A comparative evaluation of in vitro phagocytic and metabolic activities of freshly isolated bovine blood neutrophils from 26 cows was performed. Degrees of phagocytosis and nitroblue tetrazolium reduction by neutrophils were measured using zymosan, Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae, Mycoplasma bovis, Salmonella sp., and Brucella abortus opsonized with fresh bovine serum. Percent phagocytosis and nitroblue tetrazolium reduction by the neutrophils were determined by microscopic evaluation of stained coverslip smears. Individual variation among cows was found in parameters measured, but age was not a contributing factor. Phagocytosis was high (73 to 81%) for E. coli, Salmonella sp., Strep. agalactiae, and zymosan; intermediate (64%) for Staph. aureus; and low (24 to 40%) for M. bovis and B. abortus. Nitroblue tetrazolium reduction was distinctly lower than zymosan (74%) for all microorganisms. Similar percentages (32 to 41%) of nitroblue tetrazolium reduction were observed for E. coli, Staph. aureus, Strep. agalactiae, and M. bovis; intermediate (28%) was obtained for Salmonella sp.; and lowest (11%) for B. abortus.