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Journal of Dairy Science Vol. 71 No. 12 3378-3389
© 1988 by American Dairy Science Association ®
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Screening of Israeli Holstein-Friesian Cattle for Restriction Fragment Length Polymorphisms Using Homologous and Heterologous Deoxyribonucleic Acid Probes1

Eric M. Hallerman2, 3,, Alona Nave2, Morris Soller2 and Jacques S. Beckmann4

Department of Genetics, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel

ABSTRACT

Genomic DNA of Israeli Holstein-Friesian dairy cattle were screened with a battery of 17 cloned or subcloned DNA probes in an attempt to document restriction fragment length polymorphisms at a number of genetic loci. Restriction fragment length polymorphisms were observed at the chymosin, oxytocin-neurophysin I, lutropin beta, keratin III, keratin VI, keratin VII, prolactin, and dihydrofolate reductase loci. Use of certain genomic DNA fragments as probes produced hybridization patterns indicative of satellite DNA at the respective loci. Means for distinguishing hybridizations to coding sequences for unique genes from those to satellite DNA were developed. Results of this study are discussed in terms of strategy for the systematic development of large numbers of bovine genomic polymorphisms.

FOOTNOTES

1 Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel. No. 2187-E, 1987 series.

2 The Hebrew University of Jerusalem.

3 Department of Animal Science, University of Minnesota, 1988 Fitch St., St. Paul 55108.

4 The Volcani Center.






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Copyright © 1988 by the American Dairy Science Association ®.