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Journal of Dairy Science Vol. 71 No. 1 68-74
© 1988 by American Dairy Science Association ®
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Influence of Recombinant Deoxyribonucleic Acid-Derived Bovine Growth Hormone on {alpha}-Lactalbumin Production by Bovine Mammary Tissue Maintained in Athymic Nude Mice1

L. G. Sheffield

Department of Dairy Science, University of Wisconsin, Madison 53706

C. M. Eppler

American Cyanamid Company, Princeton, NJ 08540

H. A. Tucker2 and C. W. Welsch3

Department of Animal Science, and Department of Anatomy, Michigan State University, East Lansing 48824

ABSTRACT

Mammary tissue from five cows was cut into pieces approximately 3 mm2 x .2 mm and placed subcutaneously in athymic mice (10 to 12 pieces/mouse). After 30 d, all mice were injected for 10 d with 17ß-estradiol (1 µg), progesterone (1 mg), ovine prolactin (1 mg), and growth hormone (recombinant DNA-derived bovine) (1 mg). Four grafts were then removed from each mouse, mice were ovariectomized, and subsequently mice were injected daily for 7 d with hydrocortisone (.2 mg, all mice), growth hormone (0, .25, .5, or 1.0 mg), and 0 or .5 mg ovine prolactin (three mice/treatment per udder). {alpha}-Lactalbumin content of grafts was 36 ± 9 µg/mg DNA after treatment with estradiol, progesterone, growth hormone, and prolactin but before hydrocortisone, prolactin, and growth hormone treatment. Hydrocortisone treatment increased {alpha}-lactalbumin to 147 µg/mg DNA. Growth hormone plus hydrocortisone treatment increased {alpha}-lactalbumin to 391, 451, and 480 µg/mg DNA for .25, .5, and 1.0 mg/d of growth hormone, respectively. Prolactin plus hydrocortisone treatment increased {alpha}-lactalbumin to 466 µg/mg DNA. With exogenous prolactin, growth hormone increased {alpha}-lactalbumin content of grafts to 581, 647, and 689 µg/mg DNA for .25, .5, and 1.0 mg/d of growth hormone, respectively. Histological examination of tissues indicated that the effect of growth hormone was not mediated through increased epithelial area. Data suggest that bovine mammary tissue is capable of organotypic differentiation in athymic mice.

FOOTNOTES

1 Supported by American Cyanamid Co., Princeton, NJ, and National Institutes of Health Research Grant HD-17331.

2 Department of Animal Science.

3 Department of Anatomy.






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Copyright © 1988 by the American Dairy Science Association ®.