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Journal of Dairy Science Vol. 70 No. 5 1045-1053
© 1987 by American Dairy Science Association ®
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Examination of Intramammary Devices from Infected and Uninfected Mammary Quarters by Scanning Electron Microscopy1

M. J. Paape, N. J. Corlett2 and W. M. Kortum3

Milk Secretion and Mastitis Laboratory, US Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705

ABSTRACT

Polyethylene intramammary devices were removed from six infected and four uninfected mammary quarters of seven lactating cows and examined by scanning electron microscopy. Infecting organisms included Corynebacterium bovis, coagulase-negative staphylococci, and an unidentified fungus. Intramammary devices from infected quarters had amorphous material adhering to large areas of the polyethylene. Large numbers of inflammatory cells and microorganisms were found concentrated within the material. Devices from uninfected quarters had less amorphous material with few adhering inflammatory cells. The amorphous material appeared to be restricted to abraded surfaces of the polyethylene. Milk somatic cell counts in stripping milk of quarters infected with Corynebacterium bovis and coagulase-negative staphylococci before and 3 wk after removal of intramammary device averaged 1.2 x 106 and .2 x 106/ml, respectively. Results suggest that increased cell counts of infected quarters containing intramammary devices were associated with microbial colonization of the amorphous material.


FOOTNOTES

1 This research was supported by Grant Number US 447 81 from BARD—The United States-Israel Binational Agricultural Research and Development Fund.

2 Milk Marketing, Inc., Strongsville, OH 44136.

3 180 Ely Road, Petaluma, CA 94952.







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Copyright © 1987 by the American Dairy Science Association ®.