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Journal of Dairy Science Vol. 70 No. 2 225-229
© 1987 by American Dairy Science Association ®
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Phage Resistance in Streptococcus lactis ssp. diacetylactis Transconjugant SLA3.2501 and Its Derivatives

Ebenezer R. Vedamuthu and Justine M. Neville1

Microlife Technics, Sarasota, FL 34243

ABSTRACT

Phage 18-16, which was virulent for Streptococcus lactis ssp. diacetylactis SLA3.25 was used to study phage-resistant characteristics of mucoid S. lactis ssp. diacetylactis transconjugant SLA3.2501 obtained through conjugative cotransfer of pSRQ2201 (Lac-plasmid) and pSRQ2202 (Muc-plasmid) to SLA3.25 (15). Interaction of phage 18-16 with SLA3.2501 and its derivatives showed that phage resistance was not related to either the lack of phage adsorption or restriction-modification. Suppression of phage replication in SLA3.2501 and its derivatives was not completely relieved by curing of either pSRQ2201 or pSRQ2202 or both.

FOOTNOTES

1 Present address: School of Education, University of Florida, Gainesville 32611.






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Copyright © 1987 by the American Dairy Science Association ®.