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Concentrations of High Density Lipoproteins Vary Among Follicular Sizes in the Bovine¹

Department of Dairy Science, University of Wisconsin, 1675 Observatory Drive Madison 53706

² Reprint requests (608/263-7522).

ABSTRACT

This study compared lipoprotein phospholipid, cholesterol, protein, and glycosaminoglycans in the lipoprotein fraction among follicular fluid pools derived from 2633 small (<6 mm), 1702 medium (6 to 10 mm), and 491 large (11 to 20 mm) bovine follicles subdivided by estradiol-17ß concentrations. High density lipoproteins were the only lipoprotein class found in follicular fluid when apolipoprotein profile of the <1.21 g/ml density fraction was analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis and silver staining. Within a follicular size class, concentrations of lipoprotein constituents did not change among follicular fluid pools with varying estradiol-17ß concentrations. As follicular size increased, follicular fluid lipoprotein phospholipid, cholesterol, and protein significantly increased. The percentage of total follicular fluid glycosaminoglycans in the <1.21 g/ml density fraction was highest for small follicles but averaged less than 5% for all follicular size classes. Therefore, bovine follicular fluid contains only high density lipoproteins and concentrations increased with follicular maturation. That increase in high density lipoprotein could be a potentially important source of cholesterol for progesterone production after the LH surge.

¹ Research was supported by the University of Wisconsin College of Agricultural and Life Sciences, Hatch Grant 2778, and a Biomedical Research Support Grant (Project #161412) from the University of Wisconsin Graduate School.

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