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Spontaneous Release of Temperate Phage by Relysogenized Lactose-Positive Transductants of Streptococcus lactis C2¹

Department of Food Science and Nutrition, University of Minnesota, 1334 Eckles Avenue St. Paul 55108

² Corresponding author.

ABSTRACT

Transduction for lactose-fermenting ability between the lactose-positive lysogen Streptococcus lactis LM0221 and plasmid-cured, prophage-cured, lactose-negative S. lactis LM2301 resulted in the appearance of lactose-positive transductants surrounded by a zone of clearing in the lactose-negative cell lawn. By using plaque assay procedures, the zones were shown to contain bacteriophage particles, and both spontaneous release and UV induction of prophage from these transductants were demonstrated. The DNA hybridization confirmed that LM2301 did not contain the prophage in its chromosome and that the zone-producing transductant KZ1 was relysogenized by the temperate bacteriophage. Further, a 4.35 Kb EcoRI digestion fragment appeared to contain the DNA sequences for integration into the chromosome and may provide a means for stabilizing cloned DNA by effecting chromosomal insertion in LM2301 derivatives. The selection of zone-producing lactose-positive transductants of LM2301 provided a means for detecting strains relysogenized by the temperate phage induced from LM0221.

¹ Published as Paper Number 15,301 of the Scientific Journal Series of the Minnesota Agricultural Experiment Station on research conducted in part under Minnesota Agricultural Experiment Station Project Number 18-62. Supported by Hatch and General Agricultural Research funds.