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Fatty Acid Composition of Bovine Teat Canal Keratin¹

Department of Animal Sciences, University of Vermont, Burlington 05045

ABSTRACT

Keratin from 507 excised teats was pooled, separated into three samples, and analyzed for fatty acid composition of extracellular and intracellular lipids. Mean weight of keratin collected per teat canal was 8.97 mg. Extracellular lipids were recovered in petroleum ether. Intracellular lipids were extracted in chloroform:methanol (2:1, vol/vol) solution. Average keratin wet weight of extracellular and intracellular lipid fractions were 37.1 and 21.9 mg/g, respectively. Polar and nonpolar lipids were present in both fractions. Predominant fatty acids in extracellular and intracellular lipids were C₁₆, C_18:1, and C₁₈. Extracellular lipids contained greater concentrations of C₄ to C_18:1 fatty acids than did intracellular lipids. Percent of total fatty acid weight comprised by each of C₄, C₁₀, C₁₂ and C₁₄ was also significantly greater in extracellular than intracellular fractions. Intracellular lipids contained a greater concentration and percent total fatty acid content comprised by C_18:2 than extracellular lipids. Data suggest lipids accessible at the keratin interface are in part of milk fat origin.

¹ Contribution Number 605 of the Vermont Agricultural Experiment Station. Approved by the Director.

² Department of Dairy Science, Ohio Agricultural Research and Development Center, Wooster 44691.