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Walter V. Price, Cheese Research Institute, Department of Food Science, University of Wisconsin, Madison 53706
ABSTRACT
An accurate method for the quantification of L(+)-lactic acid and D(–)-lactic acid in cheese and calcium lactate crystals found on the surface of cheese was devised through the modification of an existing L(+)-lactic acid test procedure.
The mean percentage of lactic acid recovered from standard solutions was 97.7 for L(+)-lactic acid and 99.5 for D(–)-lactic acid. When known amounts of L(+)- or D(–)-lactic acid were added to calcium lactate crystals and Cheddar cheese, a high percentage of the added lactic acid was recovered, indicating that no racemization of lactic acid occurred during the assay procedure.
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