JDS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Dairy Science Vol. 69 No. 5 1246-1249
© 1986 by American Dairy Science Association ®
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Echols, R. E.
Right arrow Articles by Foster, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Echols, R. E.
Right arrow Articles by Foster, W.

Analysis of Thiamine in Milk by Gas Chromatography and the Nitrogen-Phosphorus Detector

Richard E. Echols, Robert H. Miller and William Foster

Department of Health Research, Southern University, Baton Rouge, LA 70813

ABSTRACT

Free thiamine and total thiamine in milk were analyzed by gas chromatography using a nitrogen-phosphorus detector. Sodium bisulfite was used to cleave thiamine into 4-methyl-5-(2-hydroxyethyl)thiazole, which can be extracted by chloroform. The procedure was simplified by the use of 3-(3-pyridyl)-1-propanol as an internal standard. Free thiamine was determined by cleaving the thiamine before reacting it with an enzyme that removes phosphate and allows for the quantitation of total thiamine. The validity of the procedure was demonstrated by analysis of homogenized, nonfat dry, and raw whole milk. Thiamine is reported in µ/100 ml.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1986 by the American Dairy Science Association ®.