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Department of Food Science, Cornell University, Ithaca, NY 14853
ABSTRACT
Bacteriophages added to whole milk were partially concentrated during ultra-filtration. At 4:1 retentate, phage had concentrated 2.4:1. Thermal destruction at 54°C followed first order kinetics up to 6% protein, whereafter it deviated. When allowed to grow in retentate in the presence of appropriate host, 3.5 generations of phage appeared after 12 h at 22°C compared with four generations in skim milk. In the presence of phage, lactic acid bacteria population increased to only 107 cfu/ml compared with 3 x 109 in their absence. Retentate starter prepared in the presence of phage was as active as skim milk starter prepared in the presence of phage.
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