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Journal of Dairy Science Vol. 68 No. 9 2143-2159
© 1985 by American Dairy Science Association ®
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Gene Transfer Systems and Molecular Cloning in Group N Streptococci: A Review1

Jeffery K. Kondo2 and Larry L. McKay

Department of Food Science and Nutrition, University of Minnesota, 1334 Eckles Avenue, St. Paul 55108

ABSTRACT

Plasmid deoxyribonucleic acid of group N streptococci codes for a wide variety of important metabolic properties associated with dairy fermentations. The increasing knowledge of the genetics of these bacteria has created exciting possibilities for genetically constructing improved starter strains. A review of current literature is presented on the genetics of group N streptococci, especially the development and implications of gene transfer and molecular cloning systems for further genetic studies and for strain improvement. Functional properties of plasmid deoxyribonucleic acid and gene transfer systems, which include transduction, conjugation, transformation, and protoplast fusion, are reviewed and followed by a discussion of recent advances in gene cloning.


FOOTNOTES

1 This is published as Paper No. 14148 of the Scientific Journal Series of the Minnesota Agricultural Experimental Station. The research was conducted in part under Minnesota Agricultural Experimental Station Project No. 18–62.

2 Department of Nutrition and Food Sciences, Utah State University, Logan, UT 84322.







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Copyright © 1985 by the American Dairy Science Association ®.