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Application of Van Soest Acid Detergent Fiber Method for Analysis of Shellfish Chitin¹

Agricultural Experiment Station, University of Alaska, Fairbanks 99701

ABSTRACT

The Van Soest acid detergent fiber method was compared with the modified Welinder method for chitin analysis of Alaskan crab and shrimp meals to determine whether chitin constituted a significant fibrous component of shellfish meals. In the Welinder method, chitin is determined gravimetrically following decalcification and deproteinization with .1 M ethylenediaminetetraacetic acid and 2 N sodium hydroxide, respectively, Chitin is a fibrous component of shellfish meals, and the Van Soest acid detergent fiber method is acceptable for determination of shellfish chitin.

¹ Contribution from the University of Alaska Agricultural Experiment Station. Published with the approval of the Director as Paper No. J-155, Journal Series, Alaska Agricultural Experiment Station, University of Alaska, Fairbanks. Part of M.S. thesis of senior author.

² Agricultural Experiment Station, University of Alaska, Fairbanks, AK 99701.

³ Palmer Research Center, Agricultural Experiment Station, University of Alaska, P.O. Box AE, Palmer, AK 99645.