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Journal of Dairy Science Vol. 68 No. 6 1401-1408
© 1985 by American Dairy Science Association ®
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Evaluation of the Lactic Acid Fermentation Process for Elimination of Mycobacteria from Wastelage1

T. A. McCaskey and Y. D. Wang

Department of Animal and Dairy Sciences, Alabama Agricultural Experiment Station, Auburn University, Auburn 36849

ABSTRACT

Nine cultures of mycobacteria (four avium, two bovis, one paratuberculosis, one smegmatis, one phlei) and one culture each of Salmonella typhimurium and Escherichia coli were placed in formulated rations of bovine manure, poultry litter, and corn forage. Rations were ensiled in polyethylene bags at 25°C, and survival of the cultures was ascertained. All cultures incubated in bovine manure silage and corn silage rations were eliminated by the 5th day of ensiling, but all survived in the poultry litter silage for 15 days. During ensiling the pH of bovine manure silage decreased from 6.3 to 4.1, that of corn silage from 5.0 to 3.8, and poultry litter silage from 8.2 to 5.5. High buffering capacity of litter silage was responsible for failure of the silage to achieve a low pH similar to other silages. All nine mycobacteria test cultures grew in lactic acid-acidified Dubos medium at and above pH 4.6, but none grew below pH 4.3. Salmonella typhimurium and Escherichia coli were eliminated from manure-formulated silages when the pH decreased to 4.5 or less; however, a pH of 4.3 or less was required to eliminate mycobacteria.


FOOTNOTES

1 This manuscript has been approved for publication by the director of the Alabama Agricultural Experiment Station and is designated as Journal Series No. 4-82249.







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Copyright © 1985 by the American Dairy Science Association ®.