HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Shin, S. Articles by Howe, B. Search for Related Content PubMed PubMed Citation Articles by Shin, S. Articles by Howe, B.

Whole Milk Extender with Antibiotics to Eliminate Campylobacter fetus from Frozen Bovine Semen

New York State College of Veterinary Medicine, Diagnostic Laboratory, Cornell University, Ithaca, NY 14851

ABSTRACT

Fresh bovine semen was contaminated with five strains of Campylobacter fetus at 10⁵ to 10⁶ organisms per ml of semen. The contaminated semen was processed to contain 500 units of polymyxin B, 500 units of penicillin G, and 1000 µg of streptomycin sulfate per ml, incubated at 35°C for 10 min, and added then to whole milk extender with like units of antibiotics for further processing. Both a low and high initial sperm count requiring extension rates 1:7 and 1:39 were studied. Specimens for bacterial viability were processed for culture prior to adding bacteria, 100 min after initial antibiotic treatment, prior to freezing, and after thawed semen was centrifuged and washed to remove extender and antibiotic residue. All strains of Campylobacter fetus were not viable 6 h postantibiotic treatment prior to freezing. All non-antibiotic treated controls were viable after thawing and washing. Removal of antibiotics from thawed semen by centrifugation and washing indicated that antibiotics had bactericidal rather than only bacteriostatic activities against Campylobacter fetus.

¹ Eastern Artificial Insemination Cooperative, Inc., Ithaca, NY 14850.